Postdoctoral position in reproductive and stress physiology - France
Thursday, July 2, 2020
The IFREMER is looking for a postdoctoral candidate to join a team of marine scientists investigating the reproduction, behavior and sex determination of various fish species of interest for the aquaculture industry. The candidate's main work will be to identify circulating MicroRNAs that are expressed very early in the sex differentiation process in seven marine fish species, as part of the European project MiSS.
- Participate in blood collection and extraction of plasma miRNAs from seven different marine species: Dicentrarchus labrax, Sparus aurata, Argyrosomus regius, Scophthalmus maximus, Lutjanus analis, Sciaenops ocellatus and Lobotes surinamensis.
- Be in charge of the bioinformatics part in relation to the CNRS (MGX) for the identification of miRNAs.
- Validate these markers by qPCR on fish taken randomly in the different farms and whose sex will only be known (by histology) after the qPCR analysis has been carried out.
- Conduct chronic stress experiments (exposure to high temperature) and sudden (exposure to air or other) and perform ELISA cortisol assays.
- In collaboration with INRA (LPGP), the candidate will propose and carry out experiments on the function of the miRNAs identified using the medaka or zebrafish as a model (e.g. using CRISPR/Cas9).
- Promote the results obtained in the form of publications and/or communications.
- PhD in animal physiology.
- Knowledge in the physiology of reproduction and stress.
- Experience in molecular biology (RNA extraction, RT-qPCR, CROSPR-CAS9).
- Experience in breeding and keeping fish in captivity.
- Experience with plasma miRNAs.
- Skills in bioinformatics and English.
For more information and application, visit euraxess.ec.europa.eu. Apply by July 22, 2020.
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